2L&5L Iibhotile zeNkcubeko yeSeli
● Iimpawu zeMveliso
01 USP Vl grade medical transparent polystyrene (PS) imathiriyeli.
02 Itekhnoloji yonyango lomphezulu weplasma yeVacuum, iphucula isakhono sokubambelela kwiseli, inokugqunywa ngecollagen kumphezulu ongaphakathi ngokweemfuno zabathengi.
03 imveliso eqhelekileyo ye-cGMP, ibhetshi nganye iphumelele uvavanyo lokusebenza.
04 Inyumba, akukho endotoxin, akukho mthombo wobushushu, akukho cytotoxicity.
I-05 ye-ISB yokubumba inkqubo, umlomo webhotile ulungile kwaye ujikeleze, ukutywinwa koqhagamshelwano nge-cap kungcono, kwaye intsalela yemveliso incinci.
06 Ibhotile ye-roller ye-5Lyamkela uyilo olunamanqanaba amabini ukunyusa indawo yoqhagamshelwano.Indawo yoqhagamshelwano phakathi kwebhotile ye-roller kunye nomatshini webhotile we-roller wamkela isakhiwo esinqabileyo sokwandisa i-coefficient ye-friction yebhotile ye-roller kunye nokunciphisa i-sliding phenomenon.
07 Ubukhulu obufanayo, akukho kugqwetheka ezantsi, ukunyamezela okungaphezulu kokujikeleza.
08 Imigca engqindilili kwisigqubuthelo sobuso yenza kube lula ukujijwa ngaphakathi nangaphandle.
Ukuvala inzala kwimitha.
Akukho DNase, RNase, akukho pyrogen, akukho endotoxin.
Ungcoliseko oluninzi oluqhelekileyo ngexesha lenkcubeko yeeseli kwiiflaski ze-spinner
Iiseli ezingcolileyo zihlala zidibana ngexesha lenkcubeko yeeseli.Usulelo oluninzi oluqhelekileyo lulolu hlobo lulandelayo:
1. Usulelo lwebhaktheriya
Iintsholongwane zimnyama kwaye zifana nesanti ecolekileyo phantsi kwemakroskopu eqhelekileyo eguqulweyo.Ngokuxhomekeke kwiibhaktheriya ezosulelekileyo, zinokuba neemilo ezahlukeneyo.Indawo yenkcubeko ngokuqhelekileyo inamafu kwaye ityheli, enempembelelo ebalulekileyo ekukhuleni kweeseli.Uninzi lweeseli ziyafa kwiiyure ezingama-24.
2. Usulelo lomngundo
Isiphakathi senkcubeko kwiflaski yesipinari seseli sicacile kwaye asinabumdaka phantsi kwemakroskopu eguqulweyo.Emva kweentsuku ezi-2-3 zokufukamela kwi-incubator ye-37-degree, isacacile, kodwa kukho ukungcola okuhambayo.Iiseli zisenokukhula xa i-hyphae ebonakalayo ibonwa, kodwa ukusebenza kweeseli kuyehla ngokuhamba kwexesha.
3. Usulelo lwentsholongwane
Usulelo lwentsholongwane akukho lula ukulubhaqa.Kwakungekho lutshintsho lubalulekileyo kwiiseli kunye nenkcubeko medium.Intsholongwane nayo ayibonakali phantsi kwe-microscope eguqulweyo.Uninzi lweentsholongwane zinempembelelo encinci ekukhuleni kweeseli, kwaye ezinye iintsholongwane zangaphandle zinokubangela ukuguqulwa kweeseli kunye nokuguqulwa.Usulelo lwentsholongwane lunokuphazamisa usulelo kunye nesivuno sentsholongwane efunekayo.
4. Usulelo lweMycoplasma
I-Mycoplasma ayibonakali phantsi kwe-microscope eguqulweyo.Ungcoliseko lwakwangoko, indlela yenkcubeko ayixokozeli.Ungcoliseko lwakamva luya kubangela ukujika kombala ophakathi, uthintelo lokukhula kweeseli, ukudityaniswa kweeseli, amasuntswana amancinane phantsi kwemakroskopu okanye nokufa.
Xa uhlakulela iiseli kwi-flask ejikelezayo, ingqwalasela kufuneka ihlawulwe kwi-sterility ngexesha lokusebenza.Umsebenzisi kufuneka enze umsebenzi wakhe wokubulala iintsholongwane ukuthintela ukuzala kwemithombo eyahlukeneyo yongcoliseko kunye nokuchaphazela inkqubo yenkcubeko yeeseli.
● IParameter yeMveliso
TC InyangwaIseliIibhotile zeRoller2L&5L
| NONE | Ubungakanani | Indawo yenkcubeko(cm2) | Cap | Unyumba | iipcs/ ipakethe | iipcs/case |
| LR022002 | 2 | 850 | Ikepusi yokutywina | Ewe | 2 | 40 |
| LR022005 | 5 | 1750 | Ikepusi yokutywina | Ewe | 1 | 20 |
I-Non-TC iNyangwa IseliIibhotile zeRoller 2L&5L
| NONE | Ubungakanani | Umthamo wokusebenza(ml) | Cap | Unyumba | iipcs/ ipakethe | iipcs/case |
| LR020002 | 2 | - | Ikepusi yokutywina | Ewe | 2 | 40 |
| LR020005 | 5 | - | Ikepusi yokutywina | Ewe | 1 | 20 |
