Indlela yokukhetha izinto ezisetyenziswa kwinkcubeko yeeseli ?
1. Qinisekisa indlela yokulima
Ngokweendlela ezahlukeneyo zokukhula, iiseli zohlulwe zibe ziindidi ezimbini: iiseli ezibambelelayo kunye neeseli ezimisiweyo, kwaye kukho neeseli ezinokukhula kuzo zombini ukubambelela kunye nokunqunyanyiswa, njengeeseli zeSF9.Iiseli ezahlukeneyo zineemfuno ezahlukeneyo zokusebenzisa inkcubeko yeeseli.Iiseli ezibambelelayo ngokubanzi zisebenzisa izinto ezityiwayo eziphathwa nge-TC, ngelixa iiseli ezimisiweyo azinazo iimfuno ezinjalo, kodwa izinto ezisetyenziswayo eziphathwe nge-TC nazo zifanelekile ekukhuleni kweeseli ezimisiweyo.Ukukhetha izinto ezisetyenziswayo ezifanelekileyo, indlela yokukhuliswa kweeseli kufuneka kuqala imiselwe ngokohlobo lweeseli.
2. Khetha uhlobo lwezinto ezisetyenziswayo
Izinto eziqhelekileyo ezisetyenziswa kwinkcubeko yeeseli ziquka iipleyiti zenkcubeko yeeseli, izitya zenkcubeko yeeseli, iiflaski zenkcubeko yesikwere, ibhotile yeseli, iifektri zeeseli,Iipayipi ze-serological, njalo njaloIbhotile yenkcubeko yinkcubeko evaliweyo, enokunciphisa ukungcola;ipleyiti yenkcubeko kunyepetri isityainkcubeko evulelekileyo, elungele imifuniselo yolawulo kunye nemifuniselo yegradient, kodwa kusenokwenzeka ukuba ibangele ungcoliseko lwebhaktiriya, olufuna abaqhubi abaphezulu.Ezinye izinto ezisetyenziswayo nazo kufuneka zisetyenziswe ngezixhobo ezizodwa.Umzekelo, i-cell shaker kufuneka isebenzise ukungcangcazela kwe-shaker ukwenza iiseli zinxibelelane ngcono nomoya, kwaye umzi-mveliso weeseli ezinamaleko angama-40 ufuna izixhobo ezizenzekelayo.Ngamafutshane, xa ukhetha uhlobo lwezinto ezisetyenziswayo, kufuneka ziqwalaselwe ngokubanzi ngokudibanisa neemfuno zovavanyo kunye nezinto ozikhethayo zokusebenza.
1.Multi-kakuhleinkcubeko yeseli Amacwecwe: Iifomathi zenkcubeko yeeseli ezisebenzisa iipleyiti zenkcubeko yeeseli ezisebenza kakuhle zifumana ukuthandwa kakhulu kuba ziququzelela uphononongo lwezinto ezininzi eziguquguqukayo, zinciphisa ixesha lokulinga, kwaye zonga iirejenti ezibizayo.Ukongeza kwi-high-throughput micro-plates, ii-micro-plates ezikhethekileyo ziye zaphuhliswa ukuququzelela i-3D kunye nenkcubeko yeseli ye-organotypic.
1) Inani lemingxuma
Kuxhomekeke kwinqanaba elifunekayo le-flux, kunye okanye ngaphandle koncedo lomatshini.I-6, i-12, i-24 kunye nezinye iipleyiti zenkcubeko zeseli ezingaphantsi kakuhle zinokongezwa ngesandla.Ukuya ku-96-kakuhleiipleyiti zenkcubeko yeseli, kungcono ukuba ube noncedo lwepipette yombane okanye umatshini.
2) Ubume bomngxuma
Umphantsi wequla unokukhethwa ukuba ube tyaba (F-bottom), ujikeleze (U-bottom), okanye i-tapered, kuxhomekeke kuhlobo lweseli kunye nesicelo esisezantsi.
3) Umbala wepleyiti
Umbala wepleyiti ene-perforated nayo ihambelana ngokusondeleyo nesicelo.Ukuba iiseli zijongwa ngemakroskopu yesigaba sokuchasana okanye ngeliso lenyama, ipleyiti yenkcubeko yeseli ecacileyo enezinto ezininzi inokukhethwa.Nangona kunjalo, kwizicelo ezingaphandle kokukhanya kokukhanya okubonakalayo (okufana ne-luminescence okanye i-fluorescence), imibala emininziiipleyiti zenkcubeko yeseli(ezifana nomhlophe okanye omnyama) ziyafuneka.
4) Unyango lomhlaba
Ukuba loluphi unyango lomphezulu weseli oza kulukhetha luxhomekeke ekubeni uhlakulela ukunqunyanyiswa okanye iiseli ezibambelelayo.
2.Iiflaski zenkcubeko yeeseli: Indawo yenkcubeko iqala kwi-25-225 cm², kwaye ngokubanzi iguquliwe, ilungele ukunamathela kunye nokukhula kweeseli.225cm² kunye ne-175cm²iiflaski zenkcubeko yeselizisetyenziselwa ubukhulu becala inkcubeko enkulu (efana nenkcubeko yeseli ye-monoclonal, njl. njl.), i-75cm² isetyenziswa kakhulu kuvavanyo lweeseli ngokubanzi (isicatshulwa ngokubanzi, ukulondolozwa kweeseli, iiseli zovavanyo, njl.njl.), i-25cm² isetyenziswa ngokubanzi kwi-It can zisetyenziselwa ukuvuselela iiseli okanye inkcubeko xa kukho iiseli ezimbalwa, kwaye xa usenza iiseli eziphambili, iibhotile ezininzi zingasetyenziselwa ukuphepha ukungcoliswa komnqamlezo.
3.Erlenmeyer iflaski: Xa kuthelekiswa nezinto ezisetyenziswayo ezifana neefektri zeeseli kunye nebhotile ye-cell roller, inendawo encinci yenkcubeko yeeseli kwaye sisixhobo senkcubeko yezoqoqosho.Umzimba webhotile weflask yenziwe ngepolycarbonate (PC) okanye izinto zePETG.Idizayini ekhethekileyo ye-triangular shape yenza kube lula ukuba i-pipette okanye i-cell scraper ifikelele kwikona yebhotile, okwenza umsebenzi wenkcubeko yeseli ulungele ngakumbi.Ii-erlenmeyer flaski-cap yenziwe ngezinto eziphezulu ze-HDPE, ezahlulahlulwe kwi-cap yokutywina kunye ne-cap yokuphefumula.Isivalo sokutywina sisetyenziselwa inkcubeko etywiniweyo yegesi kunye nolwelo.Icap yokuphefumla ifakwe i-hydrophobic filter membrane phezulu kwebhotile yebhotile.Ikhusela ukungena kunye nokuphuma kwe-microorganisms, ikhusela ukungcola, kwaye iqinisekisa ukutshintshiselwa kwegesi, ukwenzela ukuba iiseli okanye ibhaktheriya zikhule kakuhle.
Ubukhulu obuqhelekileyo be-conical shakeiiflaski ze-erlenmeyeryi 125ml, 250ml, 500ml, 1000ml kunye3L,5L iiflaski zeerlenmeyer ezisebenza kakuhle kakhulu, Ukuze ugcine umthamo we-medium kwaye ubambe imeko yokukhula kweeseli, isikali siya kuprintwa kumzimba webhotile.Ukukhuliswa kweeseli kufuneka kuqhutywe kwindawo engcolileyo.Ngoko ke, i-Flask ye-Erlenmeyer iya kufumana unyango olukhethekileyo lokuvala inzalo ngaphambi kokuba isetyenziswe ukufezekisa umphumo we-DNase, akukho RNase, kunye nezilwanyana eziphuma kwizilwanyana, ezibonelela ngeemeko ezintle zokukhula kweeseli.ezingqongileyo.
4.Uluhlu oluninziUmzi-mveliso weeseli: Umzi-mveliso weeseli Ufanelekile kwimveliso yebhetshi yezoshishino, ezifana nezitofu, i-monoclonal antibodies okanye i-pharmaceutical industry, kodwa kunye nokusebenza kwebhubhoratri kunye nenkcubeko yeeseli ezinkulu.Ukulungeleka kunye nokusebenza, ukuphepha ngokufanelekileyo ungcoliseko.Umzi-mveliso weSeli onesigqubuthelo esitywiniweyo: Isigqubuthelo asinamingxuma yokuphefumla, kwaye sisetyenziswa ikakhulu phantsi kweemeko ezingenakharbon diokside ezifana ne-incubators kunye nezindlu zokugcina izityalo.I-Cell Factory enekhava evaliweyo inokuthintela uhlaselo lweebhaktheriya zangaphandle kwaye incede ukudala imeko efanelekileyo yokukhula kweseli.Isigqubuthelo esiphefumlayo: Kukho imingxuma yokuphefumla kumphezulu wokhuselo, esetyenziswa ikakhulu kwindawo enekharbon diokside.Imingxuma yokuphefumla ivumela ikharbon diokside kwindawo engqongileyo ukuba ingene kumzi-mveliso weeseli, idala iimeko ezifanelekileyo zokukhula kweeseli.Kukho umaleko omnye, umaleko o-2, umaleko o-5, umaleko o-10, umaleko o-40iifektri zeseliekhoyo.
5.Inkcubeko yeseliibhotile ye-roller: Iibhotile ze-2L & 5L ze-Roller zifanelekile kwiindidi ezahlukeneyo zeeseli ezinamathele kunye neenkcubeko ze-Suspension cell, kuquka iiseli ze-Vero, iiseli ze-HEK 293, iiseli ze-CAR-T, i-MRC5, iiseli ze-CEF, i-porcine alveolar macrophages, iiseli ze-myeloma, iiseli ze-DF-1, Iiseli ze-ST, iiseli ze-PK15, iiseli ze-Marc145 ezinye iiseli ezinamathele.Ikwafanelekile kwinkcubeko ye-static yeeseli zokumiswa ezifana neeseli ze-CHO, iiseli zezinambuzane, iiseli ze-BHK21 kunye neeseli ze-MDCK.
3.Khetha iinkcukacha zezinto ezisetyenziswayo.
Imifuniselo yenkcubeko yeeseli ezinkulu ifuna izinto ezisetyenziswayo ezinommandla wenkcubeko omkhulu wokuxhasa, ngelixa iimvavanyo ezincinci zikhetha izinto ezisetyenziswayo ezinommandla omncinci.Iifektri zeeseli zisetyenziselwa ubukhulu becala inkcubeko yeeseli ezinkulu, ezifana nokuveliswa kwesitofu sokugonya, i-monoclonal antibodies, ishishini lamachiza, njl.;iipleyiti zenkcubeko, izitya, kunye neeflaski zifanelekile kwinkcubeko yeseli encinci kwiilabhoratri;ukongeza kwinkcubeko yeseli yokunqunyanyiswa, i-flask inako Ukulungiselela okuphakathi, ukuxuba kunye nokugcinwa.Ngokomlinganiselo wenkcubeko yeeseli, misela iinkcukacha ezithile zezinto ezisetyenziswayo.
Izinto ezifanelekileyo zokusebenzisa inkcubeko yeeseli zisisiseko sokuqinisekisa ukukhula okuhle kweeseli, kwaye zikwasisitshixo sokukhawulezisa inkqubo yovavanyo kunye nokuqinisekisa isiphumo senkcubeko.Kukhetho, izinto ezifana nendlela yenkcubeko yeeseli, isikali senkcubeko, kunye neemeko zelabhoratri kufuneka ziqwalaselwe ngokubanzi.kufuneka sisebenzise ezinye izinto ezityiwayo xa sisenza inkcubeko yeeseli, umzekelo,i-flake carrier yeCellDisk&umthwali ongqukuva weCellDisk,iingcebiso zepipette,ifilimu yokutywina,iipipettes, njl., uLuoron unokubonelela.
I-LuoRon Biotech Co., Ltd igxile kuphando, uphuhliso, intengiso kunye nenkonzo yezinto ezisetyenziswa kwibhayoloji.Umzi-mveliso wemveliso unommandla wesityalo oziimitha ezili-10,000 zesikwere.Inebakala le-100,000 leworkshop yemveliso ecocekileyo, iworkshop yendibano yenqanaba le-10,000 kunye nophando oluchanekileyo oluchanekileyo kunye neworkshop yemveliso.
Ngamafutshane, xa ukhetha uhlobo lwezinto ezisetyenziswayo, kufuneka ziqwalaselwe ngokubanzi ngokudibanisa neemfuno zovavanyo kunye nezinto ozikhethayo zokusebenza.Ngokuqinisekileyo, kubaluleke kakhulu ukukhetha iqonga elifana neLuoRon elinomgangatho ophezulu kunye neemveliso ezahlukeneyo, ukubonelela okuzinzile, umgangatho oqinisekisiweyo kunye nenkonzo.I-LuoRon inokubonelela ngoluhlu olupheleleyo lweenkonzo zokuthengwa kwe-one-stop kwizibonelelo zophando lwezesayensi kwiilabhoratri kwiinkalo zenzululwazi yobomi behlabathi, ishishini lamachiza, ukhuseleko lokusingqongileyo, ukhuseleko lokutya, ii-arhente zikarhulumente, kunye neyeza zonyango.
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