Inkcubeko yeeseli yindlela yokuba iiseli ziphile, zikhule, zivelise kwakhona kwaye zigcine izakhiwo zazo eziphambili kunye nemisebenzi ngokulinganisa okusingqongileyo kwi-vivo in vitro.Ibhotile yenkcubeko yeseliluhlobo lweseli esetyenziswayo edla ngokusetyenziswa kwinkcubeko yokubambelela kwiiseli.Kwinkqubo yenkcubeko yeeseli, sihlala sifumana ukuqokelelwa kokungcola okuthile kulwelo.Kukho izizathu ezininzi zale meko, kwaye ubushushu bukwasinye sezizathu eziqhelekileyo.
Ubukho bemvula kwiflaski yenkcubeko yeseli bunokuba sisiphumo sokungcoliseka kweeseli.Ukuba ungcoliseko aluqukwanga, i-turbidity kwindawo yenkcubeko yeseli idla ngokutolikwa njengemvula yezinto zetsimbi, iiproteni, kunye namanye amacandelo aphakathi.Uninzi lwe-precipitates luphazamisa ukwanda kweeseli eziqhelekileyo ngenxa yokuba ziguqula ukubunjwa kwe-medium nge-chelating izondlo kunye nezinye izinto ezifunekayo.I-precipitate inokujongwa nge-microscopically kwaye inokuphazamisa imifuniselo efuna uhlalutyo lwe-imaging.
 
Kwinkcubeko yeeseli, ubushushu yenye yezinto eziphambili ezibangela imvula.Xa ubushushu butshintsha ngokugqithisileyo, iiprotheyini zeplasma ezinobunzima obuphezulu ziya kuncitshiswa kwisisombululo.Ukungasebenzi kobushushu kunye nomjikelo wokunyibilika komkhenkce unokukhuthaza ukuthotywa kweprotheyini kunye nemvula.Ngenxa yokuba i-liquid okanye i-medium reconstituted medium igcinwa kwindawo yokugcina ebandayo phakathi kokusetyenziswa, ityuwa inokuhlala, ngakumbi kwi-10X okanye ezinye izisombululo zokugcina ezigxininisiweyo.
 
Ewe, imvula ibonakala kwibhotile yenkcubeko yeseli.Ukuba kugqitywe ukuba ubushushu bungunobangela, ingqwalasela kufuneka ihlawulwe kwindawo yokugcina kunye nendlela yokusebenza kwendawo yenkcubeko ukuphepha ukukhenkceza okuphindaphindiweyo kunye nokunyibilika, okunokunciphisa amathuba okuba nemvula.