Siyabonaiifektri zeselulakwiinkalo ukusuka kulungiselelo lwesitofu ukuya kwi-biopharmaceuticals.Yinqanawa yenkcubeko yeeseli ezinomaleko amaninzi, enoncedo lokuhlala kwindawo encinci kunye nesantya esiphezulu sokuvunwa kweeseli.Iiseli zinovakalelo kakhulu kwindawo yazo, ngoko kukho izinto ezine ekufuneka uziqwalasele xa usenza oku:

1. Xa iiseli ze-culture, yonke imisebenzi kufuneka yenziwe ngokungqongqo ngokuhambelana neemfuno zokusebenza kwe-aseptic.

2. Nceda uyifudumeze ngaphambiliumzi-mveliso weselikunye nephakathi ukuya kwiqondo lobushushu lenkcubeko kwangaphambili: Njengoko inkulu i-incubator, ixesha elide liya kuba lifikelele kwiqondo lobushushu lenkcubeko, ngoko ke phambi kovavanyo, ukutshisa umzi mveliso weseli kunye nephakathi ukuya kwiqondo lobushushu lenkcubeko kunokukhawulezisa isantya sokubambelela kwiseli kwaye phucula kakhulu izinga lokuvuna iseli.

3. umsebenzi kufuneka ube mnene, uthintele ukungcangcazela okukhulu ukuvelisa amaqamza: amaqamza anokubangela ukuba imidiya impompoze ukusuka kumaleko angaphezulu ukuya kumaleko asezantsi, okukhokelela kusasazo lwemidiya olungalinganiyo, kunye nokudityaniswa kweeseli.

4. Kuphephe ukutshiza utywala okanye isibulali ntsholongwane kwisigqubuthelo esiphefumlayo.Utywala okanye isibulala-ntsholongwane sinokunyakamisa i-membrane yesihluzi se-hydrophobic, okubangela ukuba kungabikho moya ophefumlayo, okuchaphazela ukutshintshiselana kwegesi okanye kubangele uxinzelelo olungalinganiyo ngexesha lokusebenza.

Ezi zezinye zeengxaki ekufuneka zigxininiswe kwaye zinikwe ingqalelo xa zikhula iiseli kwiumzi-mveliso weseli.Inkcubeko yeseli ngumsebenzi ongqongqo kakhulu kunye nocokisekileyo, kwaye ukungahoywa okuncinci kunokubangela ungcoliseko lweseli, ukuqhobosha, ukungabambeleli eludongeni, njl.njl. Kuphela ngokufumana indlela yokusebenza echanekileyo sinokuqinisekisa inkqubela phambili egudileyo yenkcubeko yeseli.