Inkcubeko yeeseli ikwabizwa ngokuba yitekhnoloji ye-cell cloning, yindlela ebalulekileyo yobugcisa yophando lwebhayoloji.Isishukumisi seseliyinto ekhethekileyo esetyenziswayo kwinkqubo yenkcubeko yeeseli.Yisiseko senkcubeko yeeseli ukuqonda iimpawu kunye nezilumkiso ze-cell shaker.Isishukumisi seseliyenziwe ngokubanzi ngezinto zePC ezisimahla zeBPA okanye iPETG ngenaliti, ukuzoba kunye nenkqubo yokuvuthela, ngolu hlobo lulandelayo.ng iimpawu:
1, I-2.8L kunye ne-5L ibhotile ye-shaker yeseliindawo yefilimu ephefumulayo ye-cap inkulu kuneemveliso ezifanayo, ezifanelekileyo kwinkcubeko ye-cell high density, umthamo osebenzayoinokuzaliswa ukuya kwi-60-80% yomthamo opheleleyo, umthamo ofanayo wemveliso yebhotile ye-shaker iphezulu.
2.Uyilo olusetyhula lwe-arc ye-2.Intamo yebhotile ye-8L iyindalo kakhulu.Ubungakanani bentamo abuqinisekisi kuphela indawo yokutshintshiselana ngegesi esebenzayo, kodwa iququzelele ukusebenza kwesandla esisodwa.
3. Ibhotile ye-5L ifakwe i-ergonouyilo lwemic yesiphatho, kulula ukuyishukumisa ibhotile kunye nokuhanjiswa kolwelo.
4.Imveliso esemgangathweni yebhotile iza ne-0.2μm yokucoca intsholongwane ephefumlayo.Ukongeza, i-cap yokudlulisa i-liquid inokuzikhethela ukwenza lula ukuhanjiswa kwe-aseptic yolwelo, kwaye i-cap yebhotile inokuthi yenziwe ngokwezifiso ukuze ilingane nokuhanjiswa kolwelo ngokweemfuno zenkqubo yabathengi.
I-5.Uyilo lwe-hydrophobic yefilimu yokuphefumula yebhotile ayichaphazeli ukutywinwa kunye nefuthe lokuphefumula emva kokudibana nolwelo.
Xa iUkusebenza okuphezulu kweFlask ye-Erlenmeyerkusetyenziswa, kuyimfuneko ukutshatisa i-shaker yenkcubeko yeseli.I-solvent akufanele idlule i-30% -40% ye-shaker xa isetyenziswe.Ngexesha lenkqubo yenkcubeko, ingqalelo kufuneka ihlawulwe kulawulo lwesantya.Air ibhatyi uhlobo shaker kufuneka ingqalelo ulawulo lobushushu, ibhatyi yamanzi uhlobo shaker ingqalelo kwinqanaba lamanzi.
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Ixesha lokuposa: Mar-27-2024