PETG inkcubeko ibhotile mediumyibhotile yeplastiki esetyenziswa kakhulu.Umzimba wayo webhotile ucace kakhulu, wamkela uyilo lwesikwere, ubunzima obulula, kwaye akukho lula ukwaphuka.Sisikhongozeli esilungileyo sokugcina.Izicelo zethu eziqhelekileyo zezi zintathu zilandelayo:

1. I-Serum: I-Serum inikeza iiseli ezinomsoco ezisisiseko, izinto zokukhula, iiprotheni ezibophayo, njl., Ukuphepha umonakalo womatshini kwiiseli, nokukhusela iiseli kwinkcubeko.ISerum yokugcina ixesha elide kufuneka igcinwe kwindawo yobushushu obuphantsi obuphakathi kwe -20°C ukuya ku -70°C.Ukuba igcinwe kwi-4 ° C efrijini, ngokubanzi ayikho ngaphezu kwenyanga enye.

I-2.Culture medium: I-culture medium iqulethe i-carbohydrates, izinto ze-nitrogenous, i-inorganic salts, iivithamini kunye namanzi, njl. .Indawo yokugcina indawo ephakathi yi-2 ° C-8 ° C, ikhuselwe ekukhanyeni.

3. Ii-reagents ezahlukeneyo: Ukongeza kwi-serum kunye ne-culture medium, iibhotile eziphakathi kwe-PETG zingasetyenziselwa njengezikhongozelo zokugcina izinto ezahlukeneyo ze-biological, ezifana ne-buffers, i-dissociation reagents, i-antibiotics, i-cell cryopreservation solutions, i-staining solutions, izongezo zokuKhula, njl. Ezinye zezi reagents kufuneka zigcinwe kwi -20 ° C, ngelixa ezinye zigcinwe kwiqondo lokushisa.Kungakhathaliseki ukuba yeyiphi indawo, ibhotile ephakathi inokuhlangabezana neemfuno zabo zokugcina.

PETG ibhotile medium ikakhulu isetyenziselwa ukubamba izisombululo ezintathu ngasentla.Ukuze kuququzelelwe ukubonwa okubonakalayo komthamo wesisombululo, kukho isikali kumzimba webhotile.Ezi zisombululo zingasentla zisetyenziswa ngokusisiseko kwinkcubeko yeeseli, kwaye ingqwalasela kufuneka ihlawulwe ekusebenzeni kwe-aseptic xa ukongeza.