Umzi-mveliso weeseli sisixhobo senkcubeko yeeseli, esinesixhobo senkcubeko yeeseli, esinokuqonda ubungakanani okanye uhlobo lwenkcubeko yeeseli, kwaye inokuqonda ukucandwa okuchanekileyo kweeseli, ezilungele imimandla emininzi efana neefektri zamayeza.kukho umaleko omnye wemizi-mveliso yeeseli, iileya ezi-2 zemizi-mveliso yeeseli, iileya ezi-5 & nemigangatho eyi-10 & nemigangatho engama-40 ekhoyo.

1. Emva kokuba umzi-mveliso weseli ungene kwi-liquid, umlomo webhotile uthatha idizayini yomlomo obanzi, onokuzalisa ngokukhawuleza kwaye uqokelele ulwelo, kwaye akulula ukuvelisa amaqamza omoya.Ngelo xesha, ukuyila komlomo omkhulu kukulungele ukutshintshiselana kwegesi kwaye ifanelekile kwinkcubeko yeeseli eziphezulu.

2. Umzi-mveliso weseli osemgangathweni uxhotyiswe ngeekepusi eziphefumlayo eziyi-0.2m kunye neekepusi ezingangeni moya, ezinokusetyenziswa kwiindawo ezahlukeneyo zenkcubeko.Imivalo ye-vent engasebenziyo isetyenziswa kwiindawo ezingqongileyo ze-CO2, kwaye ii-caps ezingangeni moya zingasetyenziswa kwii-incubators eziqhelekileyo kunye ne-CO2-free greenhouses.Ukongeza, i-cap ye-liquid cap inokuzikhethela, ekulungele ukutyisa ulwelo lwe-aseptic, kunye ne-cap yebhotile efanelekileyo yokondla ulwelo nayo inokwenziwa ngokwezifiso ngokweemfuno zenkqubo yomthengi.

3. Ifilimu ephefumulayo yebhotile yebhotile yenzelwe ukuba ibe yi-hydrophobic, kwaye ayiyi kuchaphazela ukunyanzeliswa komoya kunye nefuthe lomoya wefilimu yokuphefumula emva kokudibanisa nolwelo.

4. Inkqubo yokubambelela ephuma kumazwe angaphandle phakathi kweefektri zeseli inokumelana ne-1.5 PSI ukuqinisekisa ukuba uxinzelelo loluhlu ngalunye lwemveliso aluvuzi ngexesha lonke lokuvelisa.

Inkcubeko yeeseli ikwasebenza kwinkcubeko yokubambelela kwiiseli ngexesha lenkcubeko yeeseli.I-Filamentous kwaye ingena ngokukhawuleza kwisigaba sokukhula kwe-logarithmic. Ngokuqhelekileyo emva kweentsuku ezimbalwa, umgangatho wenkcubeko uhlanganiswe ukwenza i-monolayer edibeneyo yeeseli, ezifana neeseli zeVero, iiseli ze-HEK 293, iiseli ze-CAR-T, i-MRC5, iiseli ze-CEF, i-porcine alveolar macrophages. , iiseli ze-myeloma, iiseli ze-DF-1, iiseli ze-ST , iiseli ze-PK15, iiseli ze-Marc145, njl. Zonke zinamathele kwindlela yenkcubeko.