Kwinkcubeko yokumiswa kweeseli,i-cell shake flaskluhlobo lwenkcubeko yeseli olusebenzisekayo.Ukukhula kweeseli ezimisiweyo akuzange kuxhomekeke kumphezulu wezinto ezixhasayo kwaye zakhula kwimeko yokunqunyanyiswa kwindawo yenkcubeko.Sibona njani ubungakanani bolwelo ekufuneka bongezwe kwinkcubeko yokwenyani?

Iimpawu eziqhelekileyo ze-cell shaker ziquka i-125ml, i-250ml, i-500ml kunye ne-1000ml ukuhlangabezana neemfuno zobukhulu obuhlukeneyo benkcubeko yeeseli.Umzekelo, iibhotile ze-125ml kunye ne-250ml ezinomthamo omncinci zisetyenziselwa iimvavanyo ezincinci, ngelixa iinkcukacha ze-500ml kunye ne-1000ml zisetyenziselwa iimvavanyo zenkcubeko yeseli ephakathi.Xa usebenzisa olu hlobo lwezinto ezisetyenziswayo, ukungcangcazela kwe-shaker kufuneka kusetyenziswe ukunciphisa ireyithi ye-cell agglomeration kunye nokugcina imeko entle yokukhula kweeseli.Ukukhuliswa kweeseli kufuneka kuqhutywe kwindawo engcolileyo.Ke ngoko, i-flask yenkcubeko engunxantathu iya kwenziwa inzalo ngokukodwa ngaphambi kokuba isetyenziswe ukufezekisa isiphumo sokungabikho kwe-DNase, akukho enzyme ye-RNA, kwaye akukho zixhobo ziphuma kwizilwanyana, ezibonelela ngendawo entle yokukhula kweeseli.

Ngokweenkcazo ezahlukeneyo zebhotile, umthamo wokuzaliswa ocetyiswayo wezinto ezine zebhotile ukusuka phantsi ukuya phezulu yi-30ml, 60ml, 125ml, 500ml.Ngokuqhelekileyo, umthamo wesisombululo kwinkcubeko yeeseli ulawulwa malunga ne-20% -30% yomthamo opheleleyo webhotile yokugungqa, kwaye kukho umgca ocacileyo wesikali kumzimba webhotile ukuququzelela ukubonwa okubonakalayo komthamo wesisombululo. .

Oku ngasentla sisixa esicetyiswayo solwelo oludityanisiweyo kwiinkcukacha ezahlukeneyo ze-cell shaker, ezingamiselwanga.Umthamo kufuneka uqwalaselwe ngokubanzi ngokweempawu zokukhula kweeseli kunye nokuxinana kwe-inoculation, ukwenzela ukuba ugweme impembelelo yokukhula kweeseli ngenxa yobuninzi obuninzi be-liquid eyongeziweyo.