I-cell vacuolation ibhekisela ekubonakaleni kwee-vacuoles (ii-vesicles) zobukhulu obuhlukeneyo kwi-cytoplasm kunye ne-nucleus yeeseli ezihlanjululweyo, kwaye iiseli ziyi-cellular okanye i-reticular.Zininzi izizathu zale meko.Sinokunciphisa vacuolation iiseli kwiiflaski yenkcubeko yeselikancinci kangangoko ngokusebenzisa imisebenzi yemihla ngemihla.
1. Qinisekisa imeko yeseli: misela imeko yeseli ngaphambi kokuhlakulela iiseli, kwaye uzame ukukhetha iiseli ezinenani eliphezulu lesizukulwana sokulima, ukuze ugweme i-vacuoles ngenxa yokuguga kweeseli ngexesha lenkqubo yokulima.

2. Qinisekisa ixabiso le-pH ye-culture medium: qinisekisa ukufaneleka kwe-pH ye-culture medium kunye ne-pH efunwa ziiseli ukuphepha ukuchaphazela ukukhula kweeseli ngenxa ye-pH engafanelekanga.
3. Lawula ixesha lokwetyisa i-trypsin: xa i-subculture, khetha i-concentration efanelekileyo ye-trypsin kwaye ukhethe ixesha elifanelekileyo lokwetyisa lokwetyisa, kwaye uphephe amaqamza omoya amaninzi ngexesha lokusebenza.
4. Qaphela isimo seeseli nangaliphi na ixesha: Xa uhlakulela iiseli, jonga imeko yeseli kwiflask yenkcubeko yeseli nangaliphi na ixesha ukuqinisekisa ukuba iiseli zidinga izondlo ezaneleyo kwaye zigweme i-cell vacuolization ngenxa yokunqongophala kwezondlo.
5. Zama ukusebenzisa i-fetal bovine serum enomgangatho omhle kunye neendlela eziqhelekileyo, kuba i-serum enjalo ityebile kwizondlo kwaye inezinto ezimbalwa ezikhuthazayo ezingaphandle, ezinokuthi ziphephe ngokufanelekileyo iingxaki ezinjalo.
Le misebenzi ingentla inokunciphisa ukukhutshwa kweeseli kwiflask yenkcubeko yeseli.Ukongeza, iimfuno zobunyumba kufuneka ziphunyezwe ngokungqongqo ngexesha lokusebenza ukunciphisa amathuba okungcoliseka okwahlukeneyo.Ukuba iiseli zifunyenwe zingcolisekile, kufuneka zilahlwe kwangethuba ukuphepha ukuchaphazela imifuniselo elandelayo.