Centrifugesesinye sezixhobo ezisisiseko esiqhele ukusetyenziswa kwiilabhoratri, esisetyenziswa kakhulu kwiilebhu zasesibhedlele.I-Centrifuge 10 sisixhobo esiyimfuneko sokwahlula i-serum, i-precipitated cells eziphathekayo, uvavanyo lwe-PCR njalo njalo.I-centrifuge yombane ehlakaniphile inesimo esihle, umthamo omkhulu, ubungakanani obuncinci kunye nemisebenzi epheleleyo.Ineenzuzo zentsebenzo ezinzileyo, isantya esilungelelanisiweyo kunye nebhalansi yokulungelelanisa ngokuzenzekelayo, ukunyuka kweqondo lokushisa eliphantsi, ukusebenza kakuhle kunye nokusebenza okubanzi.Umbane okrelekreleicentrifugeifanelekile kuhlalutyo olusemgangathweni lwe-serum, i-plasma kunye ne-urea kwiimveliso zonyango, izikhululo zegazi, izilingo zeklinikhi kunye neelebhu ze-biochemical.

Khetha i-centrifuges eqhelekileyo, ngokobukhulu bomthwalo womsebenzi, ngokukodwa kwiinkalo ezimbini zesantya kunye nomthamo.Ezi nkcukacha zilandelayo zokuthengwa kwee-centrifuges ezichanekileyo kufuneka zithathele ingqalelo iingxaki:

1. Isantya
I-Centrifuges yahlulwe kwisantya esisezantsiiicentrifuges<10000rpm/min, isantya esiphezuluiicentrifuges10000rpm/min ~ 30000rpm/min, kunye ne-ultra-high-speed centrifuges>30000rpm/min ngokwesantya esiphezulu.I-centrifuge nganye inesantya esiphezulu esilinganiselweyo, kwaye isantya esiphezulu sibhekisela kwisantya phantsi kweemeko zokungabikho komthwalo.Nangona kunjalo, isantya esiphezulu siyahluka ngokohlobo lwe-rotor kunye nobukhulu besampula.Ngokomzekelo, isantya esilinganisiweyo se-centrifuge yi-16000rpm / min, ebonisa ukuba i-rotor ijikeleza amaxesha angama-16,000 ngomzuzu xa umthwalo ungalayishwanga, kwaye isantya ngokuqinisekileyo siya kuba ngaphantsi kwe-16000rpm / min emva kokongeza isampuli.I-rotor eyahlukileyo, isantya esiphezulu sikwahlukile;I-centrifuge evela kumazwe angaphandle ingakhethwa kunye nenani le-rotors, kwaye abavelisi abambalwa bee-centrifuges zasekhaya baye baphuhlisa ngempumelelo ubuchwepheshe obunjalo, njenge-TG16 desktop high-speed centrifuges, TGL16, TGL20 desktop high-speed centrifuges efrijini, kunye nezinye iimodeli ezininzi zinoku ilayishwe ngeentlobo ezili-16 zeerotors, ezinokusetyenziswa kumatshini omnye.I-rotor ethe tyaba inokufikelela kwi-15000rpm / min, kodwa i-Angle rotor inokufikelela malunga ne-14000rpm / min, umahluko okhethekileyo ukubonisana nabasebenzi bokuthengisa imveliso kunye nabasebenzi abafanelekileyo bezobugcisa besityalo sokuvelisa ngokweenkcukacha, ngoko ke ukhetho lwesantya kufuneka luqaphele, isantya esiphezulu se-centrifuge ekhethiweyo kufuneka sibe phezulu kunesantya esijoliswe kuyo.Ngokomzekelo, ukuba isantya esijoliswe kuyo yi-16000rpm / mIn, isantya esiphezulu se-centrifuge ekhethiweyo kufuneka sibe phezulu kune-16000rpm / min.Ngokuqhelekileyo, umphumo wokwahlula ubukhulu becala kuxhomekeke kwisantya, kodwa amandla e-centrifugal, ngoko ngamanye amaxesha isantya asihambisani neemfuno, nje ukuba amandla e-centrifugal anokufikelela kumgangatho, umfuniselo unokufezekisa umphumo oyifunayo.

I-Centrifugal force calculation formula: RCF=11.2×R× (r/min/1000) 2 R imele i-centrifugal radius, r/min imele isantya

2. Ubushushu
Ezinye iisampulu ezinje ngeeproteni, iiseli, njl.njl. ziya kutshatyalaliswa kwindawo yobushushu obuphezulu, obufuna ukuba kukhethwe umkhenkce.iicentrifuges, ezinoluhlu lobushushu obulinganisiweyo.I-Centrifuge ngesantya esiphezulu xa ubushushu buveliswa kunye ne-centrifuge yefriji yokulinganisela kwiqondo lobushushu elithile, iisampulu ze-centrifuge ngokubanzi kufuneka zigcinwe kwi-3 ° C ~ 8 ° C, isixa esithile sinokufezekiswa kunye ne-rotor, njenge-centrifuge rated. Uluhlu lobushushu -10 ° C ~ 60 ° C, faka i-rotor ethe tye inokufikelela malunga ne-3 ° C xa ijikeleza, ukuba i-rotor e-angular, inokufikelela kuphela malunga ne-7 ° C. Le ngongoma kufuneka idibane nabasebenzi bokuthengisa imveliso. kunye nabasebenzi abafanelekileyo bobugcisa bomzi-mveliso wemveliso ngokweenkcukacha.

3. Umthamo
Zingaphi iityhubhu zesampulu ekufuneka zifakwe kwi-centrifuged ngexesha?Ingakanani umthamo ofunekayo ityhubhu nganye yesampulu?
Ezi zinto zinquma umthamo opheleleyo we-centrifuge, ukuthetha ngokulula, umthamo opheleleyo we-centrifuge = umthamo wetyhubhu nganye ye-centrifugal × inani leetyhubhu ze-centrifugal, umthamo opheleleyo kunye nobukhulu bomsebenzi uhambelana.

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