Kanye iiseli sinkcubeko kuyo

umzi-mveliso weselizingcolisekile, uninzi lwayo kunzima ukuyiphatha.Ukuba iiseli ezosulelekileyo zixabisekile kwaye kunzima ukuzifumana kwakhona, ezi ndlela zilandelayo zinokusetyenziswa ukuzisusa.

1. Sebenzisa amayeza okubulala iintsholongwane

Ii-antibiotics zisebenza ngakumbi ekubulaleni iibhaktheriyaiifektri zeseli.Iyeza elidityanisiweyo lisebenza ngakumbi kuneyeza lodwa.Iyeza lothintelo lisebenza ngakumbi kunamayeza emva kosulelo.Amayeza othintelo ngokuqhelekileyo asebenzisa amayeza okubulala iintsholongwane kabini (penicillin 100u/mL plus streptomycin 100μg/mL).Emva kokungcola, indlela yokucoca kufuneka ibe ngamaxesha angama-5 ukuya kwe-10 ngaphezu kwexabiso eliqhelekileyo.Ichiza kufuneka lisetyenziswe kwiiyure ezingama-24 ukuya kwezingama-48 emva kokongezwa, kwaye litshintshwe ngokwesiqhelo.Ulwelo lwenkcubeko.Le ndlela inokusebenza kumanqanaba okuqala osulelo.Ukongeza kwi-penicillin kunye ne-streptomycin, i-antibiotics esetyenzisiweyo ingabandakanya i-gentamicin, i-kanamycin, i-polymyxin, i-tetracycline, i-nystatin, njl. Ngokuqhelekileyo isetyenziswa yi-400 ukuya kwi-800 μg / mL kanamycin okanye i-200 μg / mL tetracycline.Isixhobo sitshintshwa rhoqo ngeentsuku ezi-2 ukuya kwezi-3 kwaye sidluliselwe kwisizukulwana esi-1 ukuya kwisi-2 sonyango.Kwiminyaka yakutshanje, kuye kwaxelwa ukuba i-4-fluoro, i-2-hydroxyquinoline (i-Ciprofloxacin, i-Cip), i-Pleu-romutilin derivative (i-Pleu-romutilin derivative, i-BM-Cyclin2: i-BM-1 kunye ne-tetracycline derivative (BM-2)) I-Antibiotics i esebenzayo ekubulaleni i-mycoplasma xa isetyenziswa yodwa okanye idibene.Ezi zintathu ze-antibiotics zilungiselelwe kwizisombululo ezigxininisiweyo ze-250X kwi-PBS kwaye zigcinwe kwi -20 ° C ukuze zisetyenziswe kamva.I-concentration yokusetyenziswa kwe-Cip yi-10 μg / mL, i-BM-1 yi-10 μg / mL, kunye ne-BM-2 yi-5μg / mL.Xa usebenzisa, qala unqwenele inkcubeko engcolisekileyo, yongeza i-RPMI1640 inkcubeko yenkcubeko equkethe i-BM-1, emva koko unqwenele inkcubeko yenkcubeko emva kweentsuku ze-3, yongeza i-RPMI1640 inkcubeko yenkcubeko equlethe i-BM-2, kunye nenkcubeko yeentsuku ze-4, njalo njalo kwiintsuku ze-3 ezilandelelanayo. .imijikelo, de ingqinwe yi 33258 fluorescent staining microscopy ukuba mycoplasma iye yapheliswa, ke eqhelekileyo inkcubeko medium yongezwa inkcubeko kunye nokupasa 3-4 amaxesha.

2. Ukufudumeza unyango

Ukufaka inkcubeko ye-tissue engcolileyo kwi-41 ° C kwiiyure ze-18 inokubulala i-mycoplasma, kodwa inemiphumo emibi kwiiseli.Ngoko ke, uvavanyo lokuqala kufuneka luqhutywe ngaphambi kokuba unyango luhlolisise ixesha lokufudumala elinokuthi libulale i-mycoplasma ukuya kwinqanaba eliphezulu kwaye libe nefuthe elincinci kwiiseli.Le ndlela ngamanye amaxesha ayithembekanga.Ukuba uphathwa ngamachiza kuqala kwaye ufudunyezwe kwi-41 ° C, umphumo uya kuba ngcono.

3. Sebenzisa i-mycoplasma-specific serum

Ungcoliseko lwe-Mycoplasma lunokususwa nge-5% yomvundla i-mycoplasma immune serum (i-hemagglutination titer 1:320 okanye ngaphezulu).Ngenxa yokuba i-antibody ethile inokunqanda ukukhula kwe-mycoplasma, ijika ibe ne-negative iintsuku ezili-11 emva konyango lwe-antiserum kwaye ihlale ine-negative kwiinyanga ezi-5 kamva.i-negative.Nangona kunjalo, le ndlela inzima kwaye ayilula kwaye ayinayo imali njengokusebenzisa i-antibiotics.

4. Ezinye iindlela

Ukongeza kwezi ndlela zikhankanywe ngasentla zokususa ungcoliseko, kukho kwakhona iindlela zokugoba kunye ne-sterilization kwizilwanyana, iindlela ze-macrophage phagocytosis, iindlela zokongeza i-bromouracil kwi-contaminated.iibhotile zenkcubekokwaye emva koko ukhanyise ngokukhanya, kunye neendlela zokuhluza, njl., kodwa zonke ziyinkathazo kwaye zingasebenzi.Ngoko ke, xa ungcoliseko lwe-mycoplasma lwenzeka, ngaphandle kokuba lubaluleke kakhulu, ngokuqhelekileyo lulahlwa kwaye luhlaziywe kwakhona.

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