Xa sisebenzisa izinto ezisetyenziswa kwinkcubeko yeeseli, sihlala sidibana nengxaki yokudlula kweseli.Namhlanje, ndiza kukwabelana nawe ngokufutshane malunga nendlela yokusebenzisa iiflasks zokushukuma ezikumgangatho ophezulu kwisicatshulwa seseli.Xa sisebenzisa iiflaski ezivuthulula ngobuchule obuphezulu(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/) kwisicatshulwa seseli, zimbini iindlela onokuthi ukhethe kuzo, ezinjengokuqokelela iiseli nge-centrifugation kwaye emva koko uhambe, okanye ngqo isicatshulwa.

Indlela yokupasa ye-Centrifugal:

(1) Ukutshintshela iiseli kwiiflask esebenza kakuhle kakhulu yokugubha kunye nenkcubeko medium kumbhobho centrifuge for centrifugation.

(2) Lahla amandla amakhulu, yongeza inkcubeko entsha kwi ityhubhu ye-centrifuge kwayeipipetteukwenza ukumiswa kweseli.

(3) Bala kwaye ugalele kwiiflaski zenkcubeko entsha ngokulandelelanayo.

Ukuba indlela ethe ngqo yamkelwe, vumela iiseli ezinqunyanyisiweyo zihlale kancinane emazantsi eflaski yokuvuthulula yobuchule obuphezulu, ifunxe i-1/2 ~ 2/3 ye-supernatant, kwaye emva koko i-pipette yenze ukunqunyanyiswa kweseli phambi kokudlula.

Into ekufuneka sinikele ingqalelo kuyo ngexesha lokusebenza kukuba i-trypsin kufuneka ifudunyezwe kwangaphambili, kwaye ubushushu bumalunga ne-37 ° C.Isantya se-centrifugation kufuneka sifaneleke.Ukuba isantya siphantsi kakhulu, iiseli azikwazi ukuhlukana ngokufanelekileyo.Ukuba isantya se-centrifugation siphezulu kakhulu kwaye ixesha lide kakhulu, iiseli ziyakucudiswa, zibangele umonakalo okanye nokufa.Iiseli kufuneka zigcinwe rhoqo, kwaye ukuba ungcoliseko lufunyenwe, kufuneka lujongwe ngexesha.