Inkcubeko yokudlula kwiseli ibhekisa kwinkqubo yokwahlula-hlula inkcubeko ibe ngamalungu amancinane kwaye iphinde igalelwe kwenye isitya senkcubeko (ibhotile) ukwenzela inkcubeko eyongezelelweyo.Ukusebenza okuphezulu kwe-cell shakeryinto eqhelekileyo esetyenziswayo kwinkcubeko yeseli yokunqunyanyiswa, indlela yokusebenzisa i-shaker ephezulu yeeseli kwisicatshulwa seseli?

Ngokwendalo yazo, iiseli ezimisiweyo azibambeleli, ngoko ke akukho zi-enzymes zifunekayo ukuzisusa kumphezulu we-shaker esebenza ngokufanelekileyo.Kwilabhoratri ngokubanzi, ipaseji ethe ngqo kunye ne-centrifugal passage idla ngokusetyenziswa ukwenza ukudlula kweeseli ezinqunyanyisiweyo.Xa iiseli zibonwa ukuba zikhule ngaphezu kwama-80 ukuya kuma-90 ekhulwini (i-cell suspension iba tyheli), iiseli zilungele ukudlula.

Ukuba iiseli zikhula kakuhle phantsi kwe-microscope, kunokusetyenziswa indlela ethe ngqo.Isiphakathi kwiiflask eshukumayo esebenza kakuhle kakhuluyahlulwe ngokulinganayo yaba yiflaski entsha yenkcubeko, kunye nesixhobo esitsha songezwa.Ukuxinana kweeseli kwaqatshelwa ngosuku olulandelayo ukufumanisa ukuba ulwelo luyafuneka na.

Ukuba imeko yeseli ayilunganga, kufuneka kusetyenziswe indlela yokupasa ye-centrifugal.Okokuqala, ukumiswa kweeseli kudluliselwa kwiityhubhu ye-centrifuge, i-centrifuged kwi-1000rpm ye-5 min, emva koko i-supernatant ilahlwa, i-cell precipitates ihlakazwa ngobumnene, kwaye iiseli ziphinde zimiswe nge-fresh medium.Ekugqibeleni, isixa esifanelekileyo sokumiswa kweeseli sifunxwa ngomququ, sifakwe kwinto entshaibhotile yenkcubeko, kunye nenani elifanelekileyo le-fresh medium yongezwa.Qhubeka uhlakulela.

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