Uxinzelelo lolwelo luxinzelelo olukhuliswe ngesiphakathi senkcubeko yolwelo.Ineenzuzo zomjikelezo omfutshane wokuvelisa, iminyaka yebhaktheriya ehambelanayo, i-inoculation efanelekileyo, ifanelekile kwimveliso yefektri, njalo njalo.Yamkelwe luninzi lwabalimi.Isishukumisi seseli (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/) luhlobo lwemathiriyeli esetyenziswayo efunekayo kwinkcubeko yolwelo.La manqaku alandelayo kufuneka athathelwe ingqalelo kwinkcubeko:

1. Ukulungiswa kwendawo yenkcubeko ngokwefomula yamacandelo ahlukeneyo, kwisitya, inyibilika ngamanzi, yahlulwe kwiibhotile ezinxantathu, ngokuqhelekileyo i-250 ~ 300mL ibhotile ye-cell shake ibhotile ye-50mL yenkcubeko,500mL ibhotile engunxantathu  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/)I-100mL i-culture medium, iplagi yomqhaphu eplagi, esongiweyo yephephandaba;Ingenziwa kwakhona ngeeleya ezisi-8 zegauze 8cm×8cm yokuvala isivalo sebhotile, ngaphandle ngokutywinwa kwephepha elimdaka.
2. Indlela ye-Autoclave sterilization iyamkelwa ngokubanzi ukwenza inzala, efuna i-121℃ kunye ne-30min.Yikhuphe kwaye uyipholise malunga ne-30℃.Yibeke kwigumbi elingenazintsholongwane okanye kwibhokisi yesitofu ukuze isetyenziswe.
3. Ngokweemfuno zokusebenza kwe-aseptic, ibhotile nganye inokudityaniswa kunye ne-2 ~ 3cm2 slant strain, kwaye i-slant strain nganye inokudityaniswa neebhotile ezi-4 ~ 5.Kungcono ukuzisa i-medium kunye neentlobo ezongezelelweyo, ukwenzela ukuba zikwazi ukudada phezu kwendawo ephakathi.Emva kwe-inoculation, gubungela umlomo webhotile nge-gauze efakwe ekuqaleni, kwaye uyibophe ngokuqinileyo ngomtya.
4. Ibhotile ye-inoculated strain inokufakwa kumandlalo oshukumisayo wenkcubeko, okanye inokubekwa kwiqondo lokushisa elingaguqukiyo le-24 ~ 26 ℃ ye-48h, kwaye ke inkcubeko ye-oscillated inokuqhutywa emva kokuba i-mycelium ye-aerogenic yandiswe kwindawo yenkcubeko. .I-oscillating frequency ye-reciprocating shaker yi-80 ~ 120r / min, kunye ne-rotary shaker yi-150 ~ 220r / min.I-3 ~ 4d inokukhuliswa kwi-shaker.Ekupheleni kwenkcubeko, ngenxa yeentlobo ezahlukeneyo, inkcubeko yenkcubeko yavela ngemibala eyahlukeneyo, efana ne-oyster mushroom, i-enok mushroom inkcubeko medium yayiyellow ukukhanya;Ikhowa, i-ericius erinaceus inkcubeko yenkcubeko yayibomvu bumdaka ngebala, kunye nevumba elimnandi lekhowa;Indawo yenkcubeko yokungunda imdaka, ngqindilili kwaye iswiti.Indawo yenkcubeko ingasetyenziselwa imveliso okanye inkcubeko yokwandisa ngakumbi kuphela xa ubunzima obomileyo be-mycelium bufikelela kwi-10g/L kunye nobubanzi beepellets ze-mycelium yi-1 ~ 2mm.
 
5. Ukuveliswa kolwelo lwesibini lwesibini lwenkcubeko yolwelo oluphakathi kwenqanaba elifanayo loxinzelelo lolwelo, isikhongozeli senkcubeko kufuneka sibe sikhulu, umthamo awukho ngaphezulu kwe-3500mL.Emva kokuvala inzala kunye nokupholisa, kwafakwa iintlobo zolwelo ezivundisiweyo5L iseli shaker(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）kumlinganiselo we-5% -10% kwaye ukhuliswe kwi-shaker ngesantya esincinci.Emva kwexesha elithile lenkcubeko ye-oscillating, i-liquid strains kunye nokusabalalisa okufanayo kwe-microspheres kunye ne-fluid fermentation liquid inokufumaneka.
Isishwankathelo, xa usebenzisa i-cell shaker kwiintlobo zenkcubeko zolwelo, usebenze ngokwezi ndlela zingasentla, kwaye ubeke ingqalelo yokulawula ubushushu, ukungcangcazela rhoqo kunye nezinye izinto kwinkqubo yokuqinisekisa isiphumo senkcubeko.
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