Ukukhula kweeseli kuneemfuno ezingqongqo kwimekobume, ubushushu, ixabiso le-PH, njl., kunye nomgangatho wezinto ezisetyenziswa kwiiseli ezisetyenziswa kwinkcubeko yeeseli nazo ziyakuchaphazela ukukhula kweeseli.Umzi-mveliso weeseliyinto eqhelekileyo esetyenziswayo kwinkcubeko yeseli enamathelayo, kwaye umgangatho wayo uchatshazelwa ikakhulu zizinto ezine.

I-1, ukuveliswa kwezinto eziluhlaza: umgangatho ophezulu wezinto eziluhlaza sisiseko somgangatho ophezulu weemveliso, i-cell factory ekrwada ye-polystyrene (PS), kwaye kufuneka ihambelane neemfuno zomgangatho we-USP Class VI, igama livavanya izinto zeplastiki. kwicandelo lezonyango kunye neemveliso zemibhobho kwizicelo ze-biomedical zovavanyo olungqongqo ngakumbi, ngokuhambelana nenkcazo yezifundo zaselabhoratri ezingezizo ezonyango.

2, indawo yemveliso: iiseli zinovelwano ngakumbi kwimeko-bume yokukhula, ngoko ke kuyafuneka ukuba izinto ezisetyenziswayo mazingaqulatha i-endotoxin kunye nezinye izinto eziyingozi kwiiseli, ezibeka phambili iimfuno eziphezulu kwindawo yemveliso.Izinto ezisetyenziswayo ziya kuveliswa kwigumbi elikhethekileyo lamawaka alishumi elicocekileyo, kwaye ziya kuhlolwa ngokungqongqo (ukufunyanwa kweplankton, ibhaktiriya yentlenga kunye namasuntswana axhonyiweyo).Ulawulo lomgangatho luya kwenziwa ngokuhambelana neworkshop ye-GMP ukuqinisekisa ukuzinza kwendawo yemveliso.

I-3, ulawulo lwenkqubo yokuvelisa: oku kubhekiselele kwimveliso kwinkqubo yokuvelisa ikhonkco ngalinye kubandakanywa, njengemilinganiselo ye-injection, ubushushu benaliti, njl., Okuya kuchaphazela umgangatho wemveliso egqityiweyo.

I-4, ukuhlolwa komgangatho: ukuhlolwa komgangatho yinkqubo ebalulekileyo emva kokugqitywa kwemveliso yefektri yeeseli, izinto eziza kuvavanywa zibandakanya ukutywinwa, ukhuseleko lwebhayoloji, ukhuseleko lomzimba kunye neekhemikhali, ukuqinisekiswa kokuqinisekiswa kwemveliso, i-surface hydrophilicity, njl., ngokusebenzisa ezi mvavanyo ukufumanisa ingaba iimveliso ziyahlangabezana nemigangatho yomgangatho.

Izinto ezichaphazela umgangatho weefektri zeeseli ikakhulu ziquka le miba ingentla.Kuphela ngokulawula le miba kakuhle sinokuvelisa iimveliso eziphezulu kwaye ngaloo ndlela sinikeze indawo efanelekileyo yokukhula kweeseli.