Vuthulula inkcubeko yeflaskikwinqanaba lokuhlolwa koxinzelelo kunye nenkcubeko (uvavanyo lokulinga), iimeko zenkcubeko kufuneka zisondele kangangoko kunokwenzeka kwiimeko zenkcubeko yemveliso yokubila, umthwalo womsebenzi mkhulu, ixesha elide, ukusebenza nzima.Izinto ezichaphazela ukusebenza okuphezulu kwenkcubeko yeflask eshukumayo ubukhulu becala lobushushu benkcubeko, i-amplitude eshukumayo ye-shaker, isixa seflask eshukumayo, i-pH yendawo yenkcubeko, i-viscosity ye-medium, njl. Umngundo ezahlukeneyo ezityiwayo nayo yahlukile, uninzi lobushushu efanelekileyo ukukhula phakathi 22 ℃ kunye 30℃, ukuba inkcubeko ubushushu liphantsi kakhulu, ukukhula mycelium kade;Xa iqondo lobushushu liphezulu kakhulu, i-mycelium pellets yayikhululekile kwaye iyancipha, kwaye amandla kunye nomgangatho we-mycelium pellets wehla.

Ukushukuma rhoqo kunye nokusebenza kakuhle okuphezulu kokulayishwa kwebhotile: umngundo otyiwayo yi-aerobic fungi, inkcubeko yolwelo, ikakhulu ngokufunxa ioksijini enyibilikisiweyo kwindawo yenkcubeko.I-oksijini echithwe kwi-culture medium ichaphazeleka kakhulu yi-viscosity ye-medium, ubuninzi be-liquid kwi-container, ukuphindaphinda kwe-oscillation kunye nezinye izinto.I-frequency ye-shaking inkulu, i-flask yokugungqa incinci, i-concentration ye-medium iphezulu, i-oksijini enyibilikileyo ye-medium iphezulu, kwaye enye indlela ejikeleze iphantsi.Ngokuqhelekileyo isantya se-rotary shaker yi-180-220 RPM / min, ukuphindaphinda yi-80-120 RPM / min, i-amplitude 6-7cm.

I-Ph yenkcubeko ephakathi: I-PH yenkcubeko yenkcubeko ichaphazela ngokuthe ngqo ukufunxa izondlo, umsebenzi we-enzyme kunye nokukhula kwe-mycelial pellet.I-pH ecacileyo kufuneka ihlengahlengiswe phambi kokuvalwa inzala, uninzi lwefungi ezityiwayo kwi-pH 2.0-6.0.Ukuze kuthintelwe utshintsho olumandla lwe-PH kwindawo yenkcubeko, i-calcium carbonate, i-phosphate kunye nezinye izinto ze-buffer zihlala zongezwa kwindawo yenkcubeko.

I-viscosity ephakathi: I-viscosity ephakathi ichaphazela ngokuthe ngqo inani le-oksijini echithwe kuyo, kwaye ichaphazela ukubunjwa kweepellets ze-mycelial.Iziphumo zabonisa ukuba xa i-viscosity ye-culture medium inyuka, ububanzi be-mycelium pellets behla, inani landa, kwaye isivuno sanda.Ke ngoko, ngokweemfuno zobunzima bolwelo kububanzi beepellets ze-mycelium, i-culture medium ene-viscosity ethile kufuneka imiselwe.Inkcubeko yeseli ngumsebenzi onzima, ngakumbi xa ifuna ukukhuliswa ngoncedo lwe-shaker, efana ne-shaker ephezulu, kufuneka ithathelwe ingqalelo ngakumbi, ukwenzela ukuba kuqinisekiswe inkqubela phambili egudileyo yenkcubeko yeseli.