Umzi-mveliso weeseli yinto eqhelekileyo esetyenziswayo kwinkcubeko yeeseli ezinkulu, esetyenziswa ikakhulu kwinkcubeko yeseli enamathelayo.Ukukhula kweseli kufuna zonke iintlobo zezondlo, ngoko ke zithini?
1. Umthombo wenkcubeko
I-cellculture medium ibonelela ngeeseli kwifektri yeeseli kunye nezondlo ezifunekayo ekukhuleni, kuquka i-carbohydrates, i-amino acids, i-salts ye-inorganic, iivithamini, njl njl. Kukho iintlobo ngeentlobo zeendaba zokwenziwa ezifumanekayo kwiimfuno zesondlo zeeseli ezahlukeneyo, ezifana ne-EBSS. , Ukhozi, MEM, RPMll640, DEMM, njl.

2. Ezinye izithako ezongeziweyo
Ukongeza kwizondlo ezisisiseko ezibonelelwa ngamajelo ahlukeneyo okwenziwa, amanye amacandelo, njenge-serum kunye nezinto, kufuneka zongezwe ngokweeseli ezahlukeneyo kunye neenjongo ezahlukeneyo zenkcubeko.
ISerum ibonelela ngezinto ezibalulekileyo ezifana ne-extracellular matrix, izinto zokukhula kunye ne-transferrin, kunye ne-fetal bovine serum isetyenziswa ngokuqhelekileyo.Umlinganiselo we-serum oza kongezwa kuxhomekeke kwiseli kunye nenjongo yesifundo.I-10% ~ 20% serum inokugcina ukukhula okukhawulezayo kunye nokwanda kweeseli, ezaziwa ngokuba yindawo yokukhula;Ukuze kugcinwe ukukhula okucothayo okanye ukungafi kweeseli, i-2% ~ 5% serum inokongezwa, ebizwa ngokuba yinkcubeko yokugcina.
I-Glutamine ngumthombo obalulekileyo we-nitrogen wokukhula kweeseli kwaye idlala indima ebalulekileyo kwinkqubo yokukhula kweeseli kunye nemetabolism.Nangona kunjalo, ngenxa yokuba i-glutamine ayizinzile kwaye kulula ukuthotywa kwisisombululo, inokubola malunga ne-50% emva kweentsuku ze-7 kwi-4 ℃, ngoko ke i-glutamine kufuneka ifakwe ngaphambi kokusetyenziswa.
Ngokuqhelekileyo, imidiya eyahlukeneyo kunye ne-serum isetyenziselwa inkcubeko yeeseli, kodwa ukwenzela ukuthintela ukungcoliswa kweeseli ngexesha lenkcubeko, inani elithile le-antibiotics, njenge-penicillin, i-streptomycin, i-gentamicin, njl., nazo zongezwa kumajelo eendaba.