Inqaku elandulelayo lazisa izizathu ezininzi zokuba kutheni iiseli zingabambeleli eludongeni xa usebenzisaiiflaski zenkcubeko yeselikunye nezinye izitya.Ke kufuneka kwenziwe ntoni ukuba isiphumo senkcubeko yokubambelela kunyango ilungile?Makhe ndikunike intshayelelo emfutshane.

Ukuba ufuna isiphumo senkcubeko yokubambelela kwiseli ibe ntle, kufuneka wenze oku kulandelayo:

1. Ukwetyisa ngokuphakathi iiseli;

2. Kucetyiswa ukuba kusetyenziswe izikhongozeli zeplastiki ezilahlwayo ezifakwe phezu komhlaba ukuze kulinywe;

3. Sebenzisa i-matrix efanelekileyo ye-extracellular okanye i-attachment reagents;

4. Ukuvuselela iiseli ezintsha, sebenzisa i-serum ye-20% ngeveki yokuqala ukunceda iiseli ukuba zibuyele;

5. I-subculture inoculation kufuneka isasazwe ngokulinganayo ukuthintela ukukhula kweqela leseli;

6. Zama ukuba ungagungqi iiseli kwiiyure ezingama-24 ze-subculture, ukuze ungachaphazeli ukubambelela.