[Kopisha] I-Flask ye-Erlenmeyer emilise okwenxantathu enetywina okanye ikepusi yokuphuma komfanekiso

[Kopisha] I-Flask ye-Erlenmeyer eneMilo engunxantathu enetywina okanye ikepusi yokuphuma

Iiflaski zeplastiki ezimilise okwe-LuoRon ezinobunxantathu, eziyilelwe usetyenziso lwenkcubeko yeseli.Yenziwe ezikumgangatho ophezulu PETG kunye nezixhobo PC, ezi iiflaski enlenmeyer ilungele zombini ukubambelela kunye nokunqunyanyiswa cell inkcubeko.Ifumaneka kwiisayizi ezine - 125ml, 250ml, 500ml kunye ne-1000ml - unokufumana lula umthamo osebenza kakuhle kuvavanyo lwakho.Ubume obukhethekileyo be-triangular buvumela ukuhamba kakuhle kweendaba kunye nokuxuba okusebenzayo, ukukhuthaza ukukhula kweeseli okunempilo.Ezi bhotile ziza kunye neminqwazi engangeni moya ukuthintela ungcoliseko kunye nokuqinisekisa indawo engcolileyo.Ngokubunjwa kwayo ekuhleni, inkqubela yenkcubeko yeeseli inokujongwa ngokulula ngaphandle kokuphazamisa inkqubo.Ezi bhotile eziguquguqukayo ziyisisombululo esiphezulu senkcubeko yeeseli ezingenamthungo, ukudibanisa ukusebenza, ukuthembeka kunye nokulula.Thatha uphando lwakho kwaye ufumane inqanaba elitsha lenkcubeko yeeseli ngeeflaski zethu zeplastiki ezinxantathu.

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Iinkcukacha zeMveliso

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Erlenmeyer Shake Flask Feature

I-erlenmeyer flask, eyaziwa ngokuba yiflask ye-triangular shake, ilungele ukulima imigca yeeseli zezinambuzane ezineemfuno eziphezulu ze-oxygen.Xa kuthelekiswa nezinto ezisetyenziswayo ezifana neefektri zeseli kunye neeflaski ze-cell spinner, indawo yenkcubeko yeseli incinci kwaye sisixhobo senkcubeko yezoqoqosho..
Umzimba weflask wenziwe ngepolycarbonate (PC) okanye izinto zePETG.Idizayini ekhethekileyo yokwakheka kwenxantathu yenza kube lula ukuba i-pipettes okanye i-cell scrapers ifikelele kwikona yeflask, yenza imisebenzi yenkcubeko yeeseli ibe lula ngakumbi.Isigqoko sebhotile senziwe ngezinto eziphezulu ze-HDPE, ezahlulwe kwi-cap yokutywina kunye ne-cap breathable cap.Isivalo sokutywina sisetyenziselwa inkcubeko etywiniweyo yegesi kunye nolwelo.I-vent cap ixhotyiswe nge-membrane yokucoca i-hydrophobic phezu kwe-cap yebhotile.Ikhusela ukungena kunye nokuphuma kwe-microorganisms, ikhusela ukungcola, kwaye iqinisekisa ukutshintshiselwa kwegesi, ukwenzela ukuba iiseli okanye ibhaktheriya zikhule kakuhle.

I-flask yenkcubeko ye-triangular iqulethwe ngumzimba webhotile kunye nebhotile yebhotile .. Idizayini ekhethekileyo yoyilo phantsi kwebhotile yenza kube lula ukuba i-pipettes okanye i-cell scrapers ifikelele kwikona yebhotile, ikhulise ukulula kwemisebenzi yenkcubeko yeseli.kunye nokuzinza.Ubukhulu obuqhelekileyo beeflaski ze-triangular shake yi-125ml, 250ml, 500ml kunye ne-1000ml.Ukuze ugcine umthamo we-medium kwaye ubambe imeko yokukhula kweeseli, isikali siya kuprintwa kumzimba webhotile.Ukukhuliswa kweeseli kufuneka kuqhutywe kwindawo engcolileyo.Ngoko ke, i-Flask ye-Erlenmeyer iya kufumana unyango olukhethekileyo lokuvala inzalo ngaphambi kokuba isetyenziswe ukufezekisa umphumo we-DNase, akukho RNase, kunye nezilwanyana eziphuma kwizilwanyana, ezibonelela ngeemeko ezintle zokukhula kweeseli.ezingqongileyo.

Iiseli zikhula kancinane kwi-Erlenmeyer Flask kunye nesisombululo

Yintoni ebangela ukukhula okucothayo kweeseli kwiiflaski ze-cell shaker
Iiseli zinovelwano kakhulu kwindawo yokukhula.Xa sihlakulela iiseli, ngamanye amaxesha sidibana nokukhula okucothayo kweeseli.Siyintoni isizathu?Zininzi izizathu zokukhula okucothayo kweeseli kwi-cell shake flask, ngakumbi ngezi zizathu zilandelayo:
1. Iiseli kufuneka zilungelelaniswe ngokutsha ngenxa yotshintsho lwenkcubeko eyahlukeneyo okanye i-serum.
2. Ii-reagents zigcinwa ngendlela engafanelekanga, kwaye amanye amacandelo ayimfuneko ekukhuleni kweeseli ezifana ne-glutamine okanye izinto zokukhula kwi-culture medium ziphelile okanye azikho okanye zitshatyalalisiwe.
3. Kukho inani elincinci lokungcoliswa kwebhaktheriya okanye i-fungal kwinkcubeko kwi-cell shaker.
4. Uxinzelelo lokuqala lweeseli ezifakwe kwi-inoculated liphantsi kakhulu.
5. Iiseli ziye zandala.
6. Usulelo lweMycoplasma
Isisombululo esicetyiswayo:
1. Thelekisa ukwakheka kwendlela entsha kunye neyokuqala, kwaye uthelekise i-serum entsha kunye neserum endala ukuxhasa imifuniselo yokukhula kweeseli.Vumela iiseli ukuba ziqhelane ngokuthe ngcembe kwindlela entsha.
2. Guqula ibe yinkcubeko yenkcubeko esandul' ukulungiselelwa, okanye wongeze i-glutamine kunye nezinto zokukhula.
3. Fukamisa ngesithinteli-esingenazintsholongwane ze-anti-antibiotics kwaye ubeke indawo yesikhuseli ukuba ungcoliseko lufunyenwe.Indawo yenkcubeko kufuneka igcinwe kwi-2-8 ° C ebumnyameni.I-Serum-equlethe i-medium epheleleyo igcinwa kwi-2-8 ° C kwaye isetyenziswe kwiiveki ezi-2.
4. Ukwandisa ukugxininiswa kokuqala kweeseli ezifakwe kwi-inoculated.
5. Faka iiseli ezintsha zembewu endaweni yazo.
6. Yahlula inkcubeko kwaye ubone i-mycoplasma.Coca iqonga kunye ne-incubator.Ukuba ungcoliseko lwe-mycoplasma lufunyenwe, buyisela inkcubeko entsha.

● IParameter yeMveliso

Udidi	Inombolo yenqaku	Umthamo	Cap	Izinto eziphathekayo	Ukucaciswa kwepakethi	Ubungakanani bebhokisi
Erlenmeyer iflaski, PETG	LR030125	125ml	itywina Cap	I-PETG,Ukuvala inzala kwimitha	1pcs/pack24pack/case	31 X 21 X 22
	LR030250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR030500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR030001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
Erlenmeyer iflaski, PETG	LR031125	125ml	I-Vent Cap	I-PETG,Ukuvala inzala kwimitha	1pcs/pack24pack/case	31 X 21 X 22
	LR031250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR031500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR031001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
I-Erlenmeyer flask, iPC	LR032125	125ml	itywina Cap	IPC, ukuvala inzala ngemitha	1pcs/pack24pack/case	31 X 21 X 22
	LR032250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR032500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR032001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
I-Erlenmeyer flask, iPC	LR033125	125ml	I-Vent Cap	IPC, ukuvala inzala ngemitha	1pcs/pack24pack/case	31 X 21 X 22
	LR033250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR033500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR033001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5