I-Fluorescence Ubungakanani bePCR bokubona iisampulu ezingama-96
Njengokhetho oluyimfuneko kucazululo lobungakanani bebhayoloji yeemolekyuli, inkqubo yePCR yexesha lokwenyani isetyenziswe ngokubanzi kwiinkalo ezahlukeneyo ezifana nophando lwezenzululwazi, ukufunyaniswa kweklinikhi kunye nokuxilongwa, umgangatho kunye novavanyo lokhuseleko, kunye nezicelo zenkundla.
Inkqubo yePCR yexesha lokwenyani
Chanekile 96
Iimbonakalo
• Ukuya kuthi ga kwi-6 imijelo yokubona i-fluorescence evumela i-multiplex PCR.
• Ukunciphisa ngokufanelekileyo i-crosstalk yemibala emininzi kunye nesiphumo somphetho, akukho lungiso lwe-ROX olufunekayo ukunciphisa isampuli kunye nokusetyenziswa kwe-reagent
• Indlela entsha yokuskena kunye netekhnoloji yokwahlulwa kwemiqondiso esonjululwe lixesha ukuphucula ubuntununtunu bokubona
• Itekhnoloji yembuyekezo yeqondo lobushushu elikhethekileyo ukunciphisa "impembelelo edge"
• Isoftware esebenziseka lula
• Itekhnoloji entsha enokukhanya kwe-LED ehlala ixesha elide ibonelela ngeziphumo zokuthembeka
Iiparamitha zobuGcisa
| Inkqubo yokulawula ubushushu | |
| Umthamo wesampuli | 96 |
| Umthamo wokuphendula | 10-50 μl |
| Itekhnoloji yomjikelo we-Thermal | I-Peltier |
| Max.Izinga lokufudumeza/ lokupholisa | 6.0° C/s |
| Ukufudumeza uluhlu lobushushu | 4 – 100 °C |
| Ukuchaneka kobushushu | ± 0.2°C |
| Ubushushu obufanayo | ±0.2℃ @60℃ , ±0.3℃ @95℃ |
| Uluhlu lwesetingi yomgangatho wobushushu | 30–100°C |
| Uluhlu lwesetingi yomgangatho weqondo lobushushu | 1 – 36°C |
| Inkqubo yokufumanisa | |
| Umthombo wokukhanya wokuvuselela | I-4/6 ye-monochrome ephezulu yee-LED ezisebenza kakuhle |
| Isixhobo sokubona | PMT |
| Imowudi yokubona | Itekhnoloji yokwahlula isignali esonjululwe lixesha |
| Uchulumanco/ukubona uluhlu lwamaza obude | 455-650nm/510-715nm |
| Imijelo yeFluorescent | 4/6 amajelo |
| Idayi exhaswayo | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Uvakalelo | Ufuzo lwekopi enye |
| Isigqibo | I-1.33 igoqa ikopi umahluko wenombolo inokwahlulwa kwi-single-plex qPCR |
| Uluhlu olunamandla | Ii-odolo ezili-10 zeekopi zobukhulu |
