Ierlenmeyer shake iflasksisikhongozeli senkcubeko esikhethekileyo senkcubeko yeseli yokunqunyanyiswa, kwaye ingasetyenziselwa ukulungiselela, ukuxuba kunye nokugcina imidiya eyahlukeneyo.Le inkcubeko consumable isetyenziswa xa culturing Jurkat iiseli.

Umgca weseli yeJurkat uthatyathwe kwigazi le-peripheral lenkwenkwe eneminyaka eli-14 ubudala kwaye iyiseli yokunqunyanyiswa.Imigca yeeseli ephuma kuJurkat engenazo iijini ezithile sele ifumaneka kwiibhanki zenkcubeko yeeseli.Imigca ye-T ye-lymphocyte engafiyo isetyenziswa ikakhulu ukufunda i-acute T cell leukemia, ukubonakaliswa kweeseli ze-T, kunye nezamkeli ezahlukeneyo ze-chemokine ezisesichengeni sokungenwa yintsholongwane, ngakumbi ukubonakaliswa kwe-HIV.Inoluhlu olubanzi kakhulu lwezicelo kuphando lwezinto eziphilayo, ezifana nokusetyenziswa kweeseli zeJurkat ukufunda i-M1-RNA ye-ribonuclease P, kunye nokufundwa kwe-M1-RNA ye-anti-MHC class II activator transcriptional (CIITA) ukuvimbela ukubonakaliswa kwe-MHC yeklasi II molekyuli kumphezulu weseli.

Xa uhlakulela iiseli zeJurkat kwi-erlenmeyer shake flasks, i-RPMI1640 medium, i-10% ye-FBS iyadingeka;iqondo lokushisa lilawulwa kwi-37 ° C, i-5% ye-carbon dioxide, ixabiso le-PH 7.2-7.4, inkcubeko ye-aseptic eqhubekayo yokushisa.Sula kwaye ubulale iintsholongwane nge-75% yotywala phambi kokudluliselwa kwi-cell ultra-clean bench, khupha i-cell cryovial kwi-tank ye-nitrogen engamanzi, uyibeke ngokukhawuleza kwindawo yokuhlambela amanzi angama-37 ° C kwaye ushukumise i-cell cryotube ngokukhawuleza ukuyinyibilika ngokukhawuleza.Emva koko, emva kwe-centrifugation, ipayipi kunye nokuxuba, njl., yafakwa kwi-incubator yeseli yokulima.

Iiseli zinovelwano ngakumbi kokusingqongileyo.Xa uhlakulela iiseli zeJurkat kwi-erlenmeyer cell shake flasks, ucoceko lomntu kufuneka lwenziwe kakuhle, ii-reagents ezinyumba kufuneka zisetyenziswe, kunye nemigaqo yokusebenza kwe-aseptic kufuneka ilandelwe ukunqanda ukwazisa ibhaktheriya kunye nokuchaphazela ukukhula kweeseli.